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Addgene inc ej5 gfp
Ej5 Gfp, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 140 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pimej5gfp/pimEJ5GFP+(Plasmid+%2344026)/pm41826730-90-8-10
Average 96 stars, based on 140 article reviews
ej5 gfp - by Bioz Stars, 2026-09
96/100 stars

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Related Articles

Plasmid Preparation:

Article Title: GCN5-TADA2B in the SAGA complex provide constitutive fine-tuning control of XRCC1 recruitment and focal retention at DNA damage sites
Article Snippet: .. Specific vector constructs were purchased to measure DSBR capacity. pHPRT-DRGFP (Addgene) was used to monitor homologous recombination repair (HRR), and pimEJ5GFP (Addgene) was used for non-homologous end joining (NHEJ). .. The pCBAScel (Addgene), which expresses I-SceI endonuclease, was used to introduce a DSB at an I-SceI site located within the GFP site of the pHPRT-DRGFP or at two I-Sce1 sites flanking pgkPURO cassette which separates the pCAGGS promoter and eGFP in the pimEJ5GFP construct.

Article Title: A novel radiosensitizer α-sulfoquinovosyl-acylpropanediol (SQAP) inhibits DNA repair pathways and sensitize cells to cancer chemotherapeutic agents.
Article Snippet: .. HR and NHEJ activities were assessed using the DR-GFP reporter system25 and the EJ5-GFP reporter system26, respectively. pDRGFP and pCBASceI were gifts from Dr. Maria Jasin (Addgene plasmid #26475 and #26477). pimEJ5GFP was a gift from Dr. Jeremy Stark (Addgene plasmid # 44026). ..

Article Title: A novel radiosensitizer α-sulfoquinovosyl-acylpropanediol (SQAP) inhibits DNA repair pathways and sensitize cells to cancer chemotherapeutic agents
Article Snippet: .. HR and NHEJ activities were assessed using the DR-GFP reporter system and the EJ5-GFP reporter system , respectively. pDRGFP and pCBASceI were gifts from Dr. Maria Jasin (Addgene plasmid #26475 and #26477). pimEJ5GFP was a gift from Dr. Jeremy Stark (Addgene plasmid # 44026). ..

Construct:

Article Title: GCN5-TADA2B in the SAGA complex provide constitutive fine-tuning control of XRCC1 recruitment and focal retention at DNA damage sites
Article Snippet: .. Specific vector constructs were purchased to measure DSBR capacity. pHPRT-DRGFP (Addgene) was used to monitor homologous recombination repair (HRR), and pimEJ5GFP (Addgene) was used for non-homologous end joining (NHEJ). .. The pCBAScel (Addgene), which expresses I-SceI endonuclease, was used to introduce a DSB at an I-SceI site located within the GFP site of the pHPRT-DRGFP or at two I-Sce1 sites flanking pgkPURO cassette which separates the pCAGGS promoter and eGFP in the pimEJ5GFP construct.

Homologous Recombination:

Article Title: GCN5-TADA2B in the SAGA complex provide constitutive fine-tuning control of XRCC1 recruitment and focal retention at DNA damage sites
Article Snippet: .. Specific vector constructs were purchased to measure DSBR capacity. pHPRT-DRGFP (Addgene) was used to monitor homologous recombination repair (HRR), and pimEJ5GFP (Addgene) was used for non-homologous end joining (NHEJ). .. The pCBAScel (Addgene), which expresses I-SceI endonuclease, was used to introduce a DSB at an I-SceI site located within the GFP site of the pHPRT-DRGFP or at two I-Sce1 sites flanking pgkPURO cassette which separates the pCAGGS promoter and eGFP in the pimEJ5GFP construct.

Non-Homologous End Joining:

Article Title: GCN5-TADA2B in the SAGA complex provide constitutive fine-tuning control of XRCC1 recruitment and focal retention at DNA damage sites
Article Snippet: .. Specific vector constructs were purchased to measure DSBR capacity. pHPRT-DRGFP (Addgene) was used to monitor homologous recombination repair (HRR), and pimEJ5GFP (Addgene) was used for non-homologous end joining (NHEJ). .. The pCBAScel (Addgene), which expresses I-SceI endonuclease, was used to introduce a DSB at an I-SceI site located within the GFP site of the pHPRT-DRGFP or at two I-Sce1 sites flanking pgkPURO cassette which separates the pCAGGS promoter and eGFP in the pimEJ5GFP construct.

Article Title: A novel radiosensitizer α-sulfoquinovosyl-acylpropanediol (SQAP) inhibits DNA repair pathways and sensitize cells to cancer chemotherapeutic agents.
Article Snippet: .. HR and NHEJ activities were assessed using the DR-GFP reporter system25 and the EJ5-GFP reporter system26, respectively. pDRGFP and pCBASceI were gifts from Dr. Maria Jasin (Addgene plasmid #26475 and #26477). pimEJ5GFP was a gift from Dr. Jeremy Stark (Addgene plasmid # 44026). ..

Article Title: A novel radiosensitizer α-sulfoquinovosyl-acylpropanediol (SQAP) inhibits DNA repair pathways and sensitize cells to cancer chemotherapeutic agents
Article Snippet: .. HR and NHEJ activities were assessed using the DR-GFP reporter system and the EJ5-GFP reporter system , respectively. pDRGFP and pCBASceI were gifts from Dr. Maria Jasin (Addgene plasmid #26475 and #26477). pimEJ5GFP was a gift from Dr. Jeremy Stark (Addgene plasmid # 44026). ..

Article Title: Loss of CFIm activates YAP/TAZ and connects mRNA cleavage and polyadenylation inhibition to BRCAness
Article Snippet: The transfected cells then received different treatments for 18 hours before running through a FACSCanto II flow cytometer (BD) to determine the percentage of GFP-positive cells. .. For NHEJ reporter assay, dTAG-NUDT21 HCT116 cells were transfected with pimEJ5GFP (Addgene, #44026) and pCBASceI (Addgene, #26477) together for 24 hours. .. The transfected cells then received different treatments for 24 hours before running through a FACSCanto II flow cytometer (BD) to determine the percentage of GFP-positive cells.

Transfection:

Article Title: Targeting FZD6 creates therapeutically actionable vulnerabilities for advanced prostate cancer
Article Snippet: .. The pDRGFP (Addgene, #26475) and pimEJ5GFP (Addgene, #44076) plasmids were transfected into C4-2 cells separately using lipofectamine 3000. ..

Article Title: Targeting FZD6 creates therapeutically actionable vulnerabilities for advanced prostate cancer.
Article Snippet: .. The pDRGFP (Addgene, #26475) and pimEJ5GFP (Addgene, #44076) plasmids were transfected into C4-2 cells separately using lipofectamine 3000. ..

Article Title: Loss of CFIm activates YAP/TAZ and connects mRNA cleavage and polyadenylation inhibition to BRCAness
Article Snippet: The transfected cells then received different treatments for 18 hours before running through a FACSCanto II flow cytometer (BD) to determine the percentage of GFP-positive cells. .. For NHEJ reporter assay, dTAG-NUDT21 HCT116 cells were transfected with pimEJ5GFP (Addgene, #44026) and pCBASceI (Addgene, #26477) together for 24 hours. .. The transfected cells then received different treatments for 24 hours before running through a FACSCanto II flow cytometer (BD) to determine the percentage of GFP-positive cells.

Reporter Assay:

Article Title: Loss of CFIm activates YAP/TAZ and connects mRNA cleavage and polyadenylation inhibition to BRCAness
Article Snippet: The transfected cells then received different treatments for 18 hours before running through a FACSCanto II flow cytometer (BD) to determine the percentage of GFP-positive cells. .. For NHEJ reporter assay, dTAG-NUDT21 HCT116 cells were transfected with pimEJ5GFP (Addgene, #44026) and pCBASceI (Addgene, #26477) together for 24 hours. .. The transfected cells then received different treatments for 24 hours before running through a FACSCanto II flow cytometer (BD) to determine the percentage of GFP-positive cells.



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